The third year of the GLAM’s project is finished. A lot of advances have been reached, but of course the last details of our research need to be improved to get the project’s objectives. Here you can find a summary of the most important advances and the situation of each Work Package. We are entering our last year and GLAM is every day closer to its end!
WP1: Biomarkers, biological tools (mAbs) and biological samples
In this first WP, 10 biomarkers were selected, based on their potential diagnostic and prognostic value in bladder cancer (S100A4, S100A7, S100A8, S100A9, S100A11, S100P, Hemoglobin, SDF1, Cytokeratin 8 and CXCL1). For the specific detection of these biomarkers by the GLAM device, new mouse monoclonal antibodies were generated. Furthermore, the biomarkers have been obtained as recombinant proteins in order to use them as a standard for the calibration of both the GLAM device and the sandwich ELISA to be developed in WP5.
WP2: Microring Fabrication and Optical Characterization
Many tasks have been finalized in this WP, such as the calibration of the deposition process to achieve low-los Yb doped Al2O3 films and the fabrication of the MRRs from the doped material with a 1% Yb. Different MRR structures with all the needed specifications could be fabricated, but achieving a 1 micron width MRR is yet challenging.
Moreover, it is done the encryptation of dual Bragg gratings on MRRs provided by UT to have two gratings with different depths on two different halves of the MRR, to achieve a degeneracy braking of the resonances on each side of the bandgap. The dual gratings are fabricated with a good periodicity, but there are some problems for both gratings and the current grating periods are different from the nominal values. Also they seem to depend on the grating depth. Further work is needed to achieve better structures. Finally, the optical properties of the fabricated structures are characterized.
WP3: Laser beating layout & Micro lens coupler & Ring Resonator Sensing & Lasing
The activities in progress in this WP are the laser beating layout & micro lens coupler and the ring resonator sensing & lasing:
An Al2O3 @ 1550 nm ring resonator for sensing experiment based on the passive cavity peak shift method is designed and the rings are functionalized with the antibody according to IBEC protocol. Protein S100A4 is detected in diluted urine and no strong matrix effect is observed, like in the undiluted urine detection.
Lasing is observed in different modes, but it needs better characterization and optimization. Moreover, micro ball lens are designed and their coupling is measured. In addition, ELISA assays in urine with AS100A4, AS100A7, AS100A8, AS100A9 and AS100P antibodies have been done, to check the correct functionalization of the surface with the specific antibodies.
WP4: e-Reader Prototype
In this WP is designed the eReader, based on cartridge and beat note. A design for the Disposable Cartridge is proposed. Furthermore, the beat frequency is detected by Heterodyne detection + FFT and by power detection.
After the beat frequency value and dynamic range is acquired, it will be necessary to test and measure the dynamic range and design the eReader based on the RF acquisition chip and FPGA.
WP5: Clinical validation: Analysis & Procedures
Different tasks have been done in this WP: the biobanking protocol IRB is approved, the urine collection is ongoing and the clinical samples for GLAM testing are available but need IRB light procedure to be released for testing. Moreover, the competetive landscape is described
Furthermore, for each biomarker, a sandwich ELISA using the tools obtained in WP1 (antibodies and recombinant proteins) is being set up. These sandwich ELISAs will be useful tools to quantify the presence of the biomarkers in the urines and compare the results to those obtained by the GLAM device.
WP6: Progress & Activities: Regulatory Issues & Dissemination
In this WP, the medical device business case and health care device are already identified. The future steps in this WP are work strongly on the system demonstrator, organize further meetings with potential partners/investors with more show case capability and file the patent applications.